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目的:探讨HPV16在宫颈鳞癌发生发展中的作用及其与c-myc、p53表达产物之间的关系。方法:采用原位杂交和免疫组化方法检测了18例慢性宫颈炎、28例CIN、4例宫颈浸润性腺鳞癌和55例宫颈浸润性鳞癌HPV16 E6 DNA及其蛋白和c-myc、p53蛋白的表达情况。结果:浸润性宫颈癌HPV16 E6 DNA及其蛋白、p53蛋白阳性率明显高于慢性宫颈炎组,c-myc蛋白阳性率则明显低于慢性宫颈炎组,p53蛋白阳性率与HPV16 E6 DNA的检出率之间无负相关关系。结论:宫颈鳞癌的发生发展可能与HPV16 E6 DNA的检出及c-myc、p53蛋白的表达异常相关。
Objective: To investigate the role of HPV16 in the development of cervical squamous cell carcinoma and its relationship with the expression of c-myc and p53. Methods: HPV16 E6 DNA, its protein and c-myc were detected in 18 cases of chronic cervicitis, 28 cases of CIN, 4 cases of invasive cervical squamous cell carcinoma and 55 cases of invasive cervical squamous cell carcinoma by in situ hybridization and immunohistochemistry. Protein expression. Results: The positive rate of HPV16 E6 DNA and its protein, p53 protein in invasive cervical cancer was significantly higher than that in chronic cervicitis group, the positive rate of c-myc protein was significantly lower than that in chronic cervicitis group, the positive rate of p53 protein and HPV16 E6 DNA No negative correlation between rates. Conclusion: The occurrence and development of cervical squamous cell carcinoma may be related to the detection of HPV16 E6 DNA and the abnormal expression of c-myc and p53 protein.