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目的调查深圳市孕妇B族链球菌(group B streptococcus,GBS)携带情况,并分析其耐药性。方法对1 277名研究对象进行问卷调查和阴道拭子采样,采用K-B纸片扩散法进行药物敏感性试验,采用聚合酶链式反应(polymerase chain reaction,PCR)检测菌株的红霉素、克林霉素、四环素耐药基因。结果孕妇阴道GBS携带率为4.5%。分离得到的57株GBS均对青霉素、万古霉素、头孢曲松、利奈唑胺敏感;但四环素(80.7%)、红霉素(79.0%)、克林霉素(68.4%)耐药率较高。GBS主要携带四环素耐药基因tet M和tet O、红霉素耐药基因erm B和mef A/E,没有检出克林霉素耐药基因lin B。结论深圳孕妇分离的GBS对四环素、红霉素、克林霉素耐药率较高,为指导今后合理使用抗生素提供数据支持。
Objective To investigate the status of carriage of group B streptococcus (GBS) in Shenzhen and to analyze its drug resistance. Methods A total of 1 277 subjects were investigated by questionnaire and vaginal swabs. Drug susceptibility test was carried out by KB disk diffusion method. The erythromycin and erythromycin were tested by polymerase chain reaction (PCR) Mycotoxin, tetracycline resistance gene. Results Pregnancy vaginal GBS carrier rate was 4.5%. Fifty-seven GBS isolates were susceptible to penicillin, vancomycin, ceftriaxone and linezolid, but the resistance rates of tetracycline (80.7%), erythromycin (79.0%) and clindamycin high. GBS mainly contains tet M and tet O tetracycline resistance genes, erm B and mef A / E erythromycin resistance genes, and clindamycin resistance lin lin was not detected. Conclusion The GBS isolated from pregnant women in Shenzhen showed a high resistance rate to tetracycline, erythromycin and clindamycin, providing data support for the rational use of antibiotics in future.