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目的 为了阐明表皮生长因了受体（ＥＧＦＲ）在喉癌组织中表达的形态学定位及其表达量与非癌变组织的差别。方 法 运用免疫组化Ｓ－Ｐ染色技术检测４５例喉癌和２１例声带息肉组织中ＥＧＦＲ的表达情况。对其中２１例喉癌和癌旁正常喉 粘膜及１０例声带息肉采用逆转录聚合酶链式反应（ＲＴ－ＰＣＲ）进行ＥＧＦＲ－ｍＲＮＡ定量分析。结果 ＥＧＦＲ阳性的棕色颗粒主 要定位于细胞膜，呈阳性着色的癌细胞弥漫性分布在喉癌组织中；而在声带息肉组织中，ＥＧＦＲ阳性着色细胞则主要分布于基 底层。 ＥＧＦＲ－ｍＲＮＡ在喉癌组织（０．９６７±０．８６５）和癌旁组织（０．８６９±０．７３４）表达水平明显高于声带息肉（０．２０５±０．０４７）（Ｐ＜ ０．０５）。结论 结果提示ＥＧＦＲ的过量表达和分布异常是喉癌的特有表现。ＥＧＦＲ－ｍＲＮＡ定量测定有可能成为喉癌早期诊断 的辅助手段。
Objective To clarify the morphological location of EGFR expression in laryngeal carcinoma and its relationship with non-cancerous tissue. Methods Immunohistochemical S-P staining was used to detect the expression of EGFR in 45 cases of laryngeal carcinoma and 21 cases of vocal cord polyps. Twenty-one cases of laryngeal carcinoma, adjacent normal laryngeal mucosa and 10 cases of vocal cord polyps were used for quantitative analysis of EGFR-mRNA by reverse transcription-polymerase chain reaction (RT-PCR). Results The EGFR-positive brown granules localized mainly in the cell membrane and the positive staining cells were diffusely distributed in the laryngeal carcinoma. In the vocal cord polyps, the EGFR-positive cells mainly distributed in the basal layer. The expression of EGFR mRNA in laryngeal carcinoma (0.967 ± 0.865) and paracancerous tissues (0.869 ± 0.734) was significantly higher than that in vocal cord polyps (0.205 ± 0.047) (P <0.05). 05). Conclusion The results suggest that over-expression of EGFR and abnormal distribution of laryngeal cancer is a unique manifestation. Quantitative determination of EGFR-mRNA may become an early aid in the diagnosis of laryngeal cancer.