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目的探讨围产期低剂量氯化三丁基锡(tributylin chloride,TBTCl)暴露对子代成年雄性小鼠发育及血清、睾丸中雌激素(E2)和睾酮(T)水平的影响是否具有持续性。方法将20只妊娠6 d的SPF级昆明小鼠采用经口灌胃方式染毒0(对照,玉米油)、1、10、100μg/kg TBTCl溶液,每组5只,每天1次,染毒至哺乳期[出生后21 d(PND21)]结束。仔鼠断乳后每周称量一次体重。至PND 84时,每组随机抽取10只雄性仔鼠,测定脾脏、肝脏、肾脏、胸腺的重量,并计算脏器系数;测定血清和睾丸匀浆组织中E2和T水平。结果与对照组比较,100μg/kg TBTCl组子代成年雄性小鼠在PND0、49、56、63、70、77时的体重均显著降低(P<0.05,P<0.01),PND84时各剂量TBTCl染毒组体重均无显著改变;脾脏重量及其脏器系数均显著升高(P<0.05),而肾脏重量及其脏器系数均显著降低(P<0.05),而肝脏和胸腺重量及其脏器系数均无显著变化;100μg/kg TBTCl染毒组睾丸组织匀浆中E2水平显著降低(P<0.05),而血清中E2和T水平及睾丸组织匀浆中T水平均无显著变化。结论围产期低剂量TBTCl暴露对子代成年雄性小鼠的发育和睾丸组织中的E2水平具有持续的毒性作用。
Objective To investigate whether prolonged perinatal exposure to low doses of tributyltin chloride (TBTCl) can affect the development of adult male mice and the levels of estrogen (E2) and testosterone (T) in serum and testis. Methods Twenty Kunming mice of SPF grade at 6 d of gestation were exposed to orally 0 (control, corn oil), 1, 10, 100 μg / kg TBTCl solution for 5 days, once a day, Until lactation [after 21 days of life (PND21)]. The weaning rats weighed once a week after weaning. At PND 84, 10 male offspring were randomly selected from each group. The weights of spleen, liver, kidney and thymus were measured and the organ coefficient was calculated. The levels of E2 and T in serum and testicular homogenate tissue were measured. Results Compared with the control group, the body weight of adult male mice in the 100 μg / kg TBTC1 group at PND0, 49, 56, 63, 70 and 77 were significantly decreased (P <0.05, P <0.01) The weight of the spleen and its organ coefficient were significantly increased (P <0.05), while the weight of the kidney and its organ coefficient were significantly decreased (P <0.05), while the weight of the liver and thymus There was no significant change in organ coefficient. The level of E2 in testis homogenate of 100μg / kg TBTCl group was significantly decreased (P <0.05), while the level of E2 and T in serum and the level of T in testis homogenate did not change significantly. Conclusions Perinatal low dose TBTC1 exposure has a sustained toxic effect on the development of adult male mice and E2 levels in testis tissue.