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【目的】利用分子标记和回交自交试验初步探讨甘蓝型油菜隐性细胞核雄性不育材料7-7365AB(Bnms 3ms 3ms 4ms 4RfRf/BnMs 3ms 3ms 4ms 4RfRf)育性的遗传模式。【方法】以纯合两型系7-7365AB为基本材料,分别构建了BnMs4位点和BnRf位点的近等基因系7-736512AB和7-7365AC,利用AFLP与BSA相结合的方法筛选与BnMs4连锁的分子标记,进行BnMs4的图谱定位;利用育性分离群体进行杂交、回交和自交,验证两基因之间的关系;利用2个基因紧密连锁的分子标记分析了176份材料的基因型。【结果】获得13个与BnMs4连锁的AFLP标记和4个SCAR标记,将该基因定位在N7连锁群的上端,与BnRf为同一个区段,并从图谱上获得CNU063、ENA06和sR4047这3个SSR标记,鉴定出了CNU063、ENA06、sR4047、SC25、SC916和SSR1这6个与BnRf共同的分子标记,它们分布于这2个基因两侧,且包含BnMs4和BnRf最短遗传图距分别为1.0cM和2.4cM。回交和自交试验证实BnMs4与BnRf并不是自由组合的遗传模式,而且几种基因型在176份材料中的分布频率也没有表现连锁不平衡现象。【结论】BnMs4和BnRf很可能属于复等位基因。
【Objective】 The genetic model of fertility in BnMS 3ms 3ms 4ms 4RfRf / BnMs 3ms 3ms 4ms 4RfRf was established by using molecular markers and backcross selfing tests. 【Method】 The homozygous 7-7365AB was used as the basic material to construct the near-isogenic lines 7-736512AB and 7-7365AC of BnMs4 and BnRf, respectively. The sequences of AFLP and BSA were screened for linkage with BnMs4 The molecular marker of BnMs4 was used to map the BnMs4 loci. The fertility segregation population was used for hybridization, backcrossing and selfing to verify the relationship between the two genes. The genotypes of 176 materials were analyzed by using two closely linked molecular markers. 【Result】 Thirteen AFLP markers and four SCAR markers linked to BnMs4 were obtained. The gene was located at the top of the N7 linkage group and was identical to BnRf. Three of CNU063, ENA06 and sR4047 were obtained from the map SSR markers were used to identify six common molecular markers CNU063, ENA06, sR4047, SC25, SC916 and SSR1, which are located on both sides of the two genes and the shortest genetic distances between BnMs4 and BnRf were 1.0 cM And 2.4cM. Backcross and selfing experiments confirmed that BnMs4 and BnRf are not a combination of genetic model, and the distribution frequency of several genotypes in 176 materials did not show the phenomenon of linkage disequilibrium. 【Conclusion】 BnMs4 and BnRf are likely to belong to the complex allele.