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目的:比较研究小鼠经中子及γ线照射后肠组织中TGF-β3的表达及意义。方法:350只二级雄性BALB/c小鼠,经不同剂量的中子和γ线照射,于照后6、12h,1、2、3、4、5、7、10、14、21及28d分批活杀,采用免疫组织化学和图像分析等技术,定量研究TGF-β3在肠组织中的动态变化规律。结果:2.5Gy照后2d内,TGF-β3表达进行性减少,阳性部位见于绒毛上皮细胞和隐窝细胞浆内;3~7d明显增加,5d达高峰;7d后逐渐恢复至正常水平。4.0,5.5Gy中子及12Gyγ线照射后4d内,TGF-β3表达进行性减少;γ线5.5Gy照后6h,TGF-β3表达反应性增加;12h~1d减少,2~5d增多,3d达高峰;5d后逐渐恢复至正常水平。结论:中子和γ线照射后,肠内源性TGF-β3的表达具有不同的变化规律,参与了肠放射损伤及修复的病理过程。
OBJECTIVE: To comparatively study the expression of TGF-β3 and its significance in intestinal tissue after neutron and γ-ray irradiation in mice. Methods: Three hundred and fifty male BALB / c mice were irradiated with different doses of neutron and γ ray at 6,12h, 1,2,3,4,5,7,10,14,21 and 28d Batch kill, using immunohistochemistry and image analysis techniques to quantitatively study the dynamic changes of TGF-β3 in the intestine. Results: Within 2 days after 2.5Gy irradiation, the expression of TGF-β3 decreased progressively, and the positive site was found in villus epithelial cells and crypt cytoplasm. The levels of TGF-β3 increased significantly from 3 to 7 days and reached the peak on 5 days. 4.0, 5.5Gy neutron and 12Gyγ ray within 4d, the expression of TGF-β3 decreased progressively; 6h after γ-ray 5.5Gy, the expression of TGF-β3 increased reactivity; decreased from 12h to 1d, increased from 2 to 5d, Peak; 5d gradually returned to normal levels. Conclusion: The changes of endogenous TGF-β3 expression after neutron and γ-ray irradiation have different rules and are involved in the pathological process of intestinal radiation injury and repair.