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目的 探讨增食因子A和B在肥胖抵抗大鼠食欲调节中的作用。方法 5 0只健康雄性SD大鼠 ,随机分为对照组和高脂组 ,分别用基础饲料和高脂饲料喂养 13周 ,然后根据体重和能量摄入量筛选出饮食诱导肥胖抵抗(diet -inducedobesityresistance ,DIO -R)和饮食诱导肥胖 (diet-inducedobesity ,DIO)组 ,观察摄食量的变化 ,Western Blot法测定大鼠脑组织中增食因子A和B的蛋白含量。结果 DIO -R大鼠总摄食量明显低于DIO大鼠 (P <0 0 5 ) ;高脂饲料可增加大鼠脑组织中增食因子A和B的含量 ,但DIO -R大鼠和DIO大鼠间无显著性差异。结论 DIO -R大鼠体内增食因子A(orexinA)和B(orexinB)的增食作用可能被其它抑制食欲因素的作用所掩盖。因而 ,orexinA和orexinB在肥胖抵抗大鼠的食欲调节中作用较弱
Objective To investigate the role of dietary factors A and B in the regulation of appetite in obesity-resistant rats. Methods Fifty healthy male Sprague-Dawley rats were randomly divided into control group and hyperlipidemic group, fed with basic diet and high-fat diet for 13 weeks, then diet-induced obesity resistance (diet-induced obesity resistance) was screened according to body weight and energy intake , DIO-R) and diet-inducedobesity (DIO). The content of dietary protein A and B in rat brain were determined by Western Blot. Results The total food intake of DIO-R rats was significantly lower than that of DIO rats (P <0.05); high-fat diet increased the content of dietary factor A and B in rat brain, but DIO-R rats and DIO No significant difference between rats. Conclusions The augmentation of orexinA and orexinB in DIO-R rats may be masked by other suppressive factors. Thus, orexinA and orexinB are less effective in the regulation of appetite in obesity resistant rats