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目的研究重组人促红细胞生成素(recombinant humane rythropoietin,rhEPO)预处理对帕金森病模型中黑质多巴胺神经元的影响及其作用的机制。方法6-羟多巴胺浸泡大鼠黑质脑片建立离体帕金森病模型,用不同浓度rhEPO在不同时间点预处理黑质多巴胺神经元,观察黑质中酪氨酸羟化酶及氧化应激指标—丙二醛、谷胱甘肽、超氧化物歧化酶和谷胱甘肽过氧化物酶的变化。结果rhEPO预处理能抑制6-羟多巴胺导致的黑质酪氨酸羟化酶阳性细胞数减少,6μ/ml rhEPO预处理在3h与6h时间点的作用优于1μ/mlrhEPO预处理(P<0.01,P<0.05);与6-羟多巴胺组相比,6μ/ml rhEPO预处理组的丙二醛值降低,谷胱甘肽、超氧化物歧化酶、谷胱甘肽过氧化物酶值增高。结论rhEPO预处理对黑质多巴胺神经元具有保护作用,其机制可能与氧化应激反应有关。
Objective To investigate the effect of rhEPO pretreatment on substantia nigra dopamine neurons in Parkinson’s disease model and its mechanism. Methods 6 - hydroxydopamine soaked brain slices of rat substantia nigra were used to establish a model of Parkinson ’s disease in vitro. The dopamine neurons were pretreated with different concentrations of rhEPO at different time points to observe tyrosine hydroxylase in substantia nigra and oxidative stress Indicators - Changes in malondialdehyde, glutathione, superoxide dismutase and glutathione peroxidase. Results RhEPO pretreatment inhibited the decrease of tyrosine hydroxylase positive cells induced by 6-hydroxydopamine, and the effect of 6μ / ml rhEPO pretreatment was better than that of 1μ / ml rhEPO pretreatment at 3h and 6h (P <0.01) , P <0.05). Compared with 6-hydroxydopamine group, the pretreatment with 6μ / ml rhEPO reduced the malondialdehyde value and the glutathione, superoxide dismutase and glutathione peroxidase . Conclusion rhEPO preconditioning can protect the substantia nigra dopamine neurons, and its mechanism may be related to oxidative stress.