论文部分内容阅读
目的 :探讨热化疗对人结肠癌LoVo细胞株中一氧化氮 (NO)含量的影响及其作用机制。方法 :实验分为对照组 ,单纯加热组、单纯化疗组及热化疗组 ,处理后用铜 镉还原法测定各级培养基中的NO含量。结果 :对照组NO含量 (45 91± 16 12 ) μmol/L ,明显低于热化疗组 (91 31± 2 4 30 ) μmol/L和单纯加热组 (6 7 6 7± 15 48) μmol/L ,P值分别 <0 0 1及 <0 0 5 ;热化疗组和单纯加热组明显高于单纯化疗组 (47 0 3± 16 0 3) μmol/L ,P值分别 <0 0 1及 <0 0 5 ;热化疗组高于单纯加热组 (P <0 0 5 ) ;单纯化疗组高于对照组 ,但无统计学意义。结论 :在肿瘤的热化疗中 ,热疗联合化疗和单纯加热能明显地增加LoVo细胞所致高浓度的NO ,单纯化疗也可以增加NO浓度 ,其机制可能是这些因素激活肿瘤细胞内NO合成酶 (NOS)产生过高浓度的NO ,产生细胞毒作用。这可能将成为热化疗肿瘤的机制之一。
Objective: To investigate the effect of thermochemotherapy on nitric oxide (NO) content in human colon cancer LoVo cell line and its mechanism of action. Methods : The experiment was divided into control group, simple heating group, chemotherapy group and thermochemotherapy group. After treatment, the content of NO in culture medium was determined by copper cadmium reduction method. Results: The NO content in the control group was (45 91± 16 12) μmol/L, which was significantly lower than that of the thermochemotherapy group (91 31 ± 2 4 30) μmol/L and the heating group alone (6 7 6 7± 15 48) μmol/L. P values were <0 01 and <0 05, respectively; the thermochemotherapy group and the simple heating group were significantly higher than the chemotherapy alone group (47 0 3 ± 16 0 3) μmol/L, P values were <0 0 1 and <0, respectively. 0.5; thermochemotherapy group was higher than the simple heating group (P <0 05); chemotherapy group was higher than the control group, but there was no statistical significance. Conclusion: In the thermochemotherapy of tumors, hyperthermia combined with chemotherapy and heating alone can significantly increase the high concentration of NO induced by LoVo cells, and chemotherapy alone can also increase the concentration of NO. The mechanism may be that these factors activate NO synthetase in tumor cells. (NOS) produces excessively high concentrations of NO and produces cytotoxic effects. This may be one of the mechanisms of thermochemotherapy tumors.