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目的探讨2-甲氧基雌二醇(2-ME)对骨髓增生异常综合征SKM-1细胞增殖和细胞凋亡的影响。方法常规培养的SKM-1细胞分为2-ME处理组与非处理对照组,SKM-1细胞分别与不同浓度2-ME(1、2、4和8μmol/L)和对照组共同培养,MTT法检测2-ME对SKM-1细胞增殖抑制作用;光学显微镜观察瑞氏-姬姆萨染色后的细胞形态;流式细胞术分析细胞周期和凋亡;荧光探针JC-1检测细胞线粒体膜电位(ΔΨm)。结果 2-ME对SKM-1细胞的生长抑制作用具有浓度和时间依赖性;光学显微镜下可见典型的凋亡细胞形态特征;SKM-1细胞被阻滞于G2/M期,表现为G0/G1期细胞和S期细胞逐渐减少,G2/M期细胞逐渐增多(P<0.05);2-ME降低细胞ΔΨm具有时间依赖性(P<0.05)。结论 2-ME对人骨髓增生异常综合征SKM-1细胞的增殖具有显著的抑制作用并可诱导细胞凋亡。其机制可能与细胞G2/M期阻滞和ΔΨm下调有关。
Objective To investigate the effect of 2-methoxyestradiol (2-ME) on the proliferation and apoptosis of myelodysplastic syndrome SKM-1 cells. Methods SKM-1 cells cultured in conventional manner were divided into 2-ME group and untreated control group. SKM-1 cells were co-cultured with 2-ME (1, 2, 4 and 8 μmol / L) The inhibitory effect of 2-ME on the proliferation of SKM-1 cells was detected by light microscopy. The Wright-Giemsa staining was observed by light microscopy. The cell cycle and apoptosis were analyzed by flow cytometry. The mitochondrial membrane potential Potential (ΔΨm). Results The growth inhibitory effect of 2-ME on SKM-1 cells was concentration-dependent and time-dependent. The morphological features of typical apoptotic cells were observed under light microscope. SKM-1 cells were arrested in G2 / M phase and showed G0 / G1 The number of senescent cells and S phase cells gradually decreased, and the number of G2 / M phase cells gradually increased (P <0.05). The 2-ME decreased the cell ΔΨm in a time-dependent manner (P <0.05). Conclusions 2-ME can significantly inhibit the proliferation of human myelodysplastic syndrome SKM-1 cells and induce apoptosis. The mechanism may be related to cell G2 / M arrest and down-regulation of ΔΨm.