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白粉病已成为威胁我国小麦稳产的重要病害之一 ,寻找并使用新的白粉病抗源成为当今抗白粉病育种的关键。报道野生二粒小麦抗白粉病基因向普通小麦转移 ,无论是正交还是反交 ,用普通小麦作轮回亲本 ,随着回交代数增加 ,抗性单株选择难度加大 ,从正、反单交F6中均获得了稳定抗病株系。同时 ,研究利用 RAPD方法对小麦—野生二粒小麦抗白粉病和感白粉病 9个衍生系进行了分析研究。研究结果表明 ,5个随机引物 ( OPH- 0 7、OPQ- 0 8、OPQ- 1 6、OPQ- 1 9、OPZ- 1 6)能在抗、感 9个衍生系中分别扩增出普通小麦所没有的野生二粒小麦特异带型 ;其中 OPH- 0 7340 bp和 OPQ- 1 990 0 bp为抗病系的特征带。
Powdery mildew has become one of the most important diseases threatening the stable yield of wheat in our country. To find and use new powdery mildew resistant sources has become the key to resistance to powdery mildew. It was reported that the resistance to powdery mildew of wild double-grain wheat transferred to common wheat, whether orthologous or reciprocal, with common wheat as the recurrent parent, with the backcross generation increased, the selection of resistant plants more difficult, from positive and negative F6 cross were obtained stable disease-resistant lines. In the meantime, 9 derivative lines of wheat-wild double grain wheat resistant to powdery mildew and powdery mildew were studied using RAPD method. The results showed that five random primers (OPH- 0 7, OPQ-0 8, OPQ-1 6, OPQ- 1 9, OPZ- 1 6) were able to amplify common wheat There is no wild type of wheat specific bands; OPH 0 7340 bp and OPQ-1 990 0 bp resistance bands characteristic bands.