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目的 研究NSAIDs的脊髓镇痛作用及与PGs、NO、EAA的关系。方法 选用Asp、Ind、KT、KPF并采用热水缩尾法、福尔马林试验、冰醋酸扭体法。结果 在热痛模型上 ,10～ 10 0ngPGE1可使小鼠痛阈显著下降。福尔马林试验 ,1μgPGE1可显著抑制其Ⅰ、Ⅱ相反应 ,10 0ng可抑制其Ⅰ相反应 ,而0 1、10ng无作用。 10 0、10 0 0ngPGE1可降低小鼠扭体次数 ,呈镇痛作用 ,而 0 1、10ng无明显效果。热痛模型上 ,NSAIDs脊髓与全身给药的有效剂量比为 1∶2 0。每只小鼠ith 10、10 0 0ngPGE1可以拮抗NSAIDs的镇痛作用。而在福尔马林和扭体试验中 ,ithPGE1不能拮抗其作用。扭体试验中 ,ithL Glu( 1mg·kg- 1)和L Arg( 0 2mg·kg- 1)预处理可以翻转NSAIDs的镇痛作用。结论 在脊髓水平 ,PGE1对不同性质疼痛调控作用不同。NSAIDs对不同性质疼痛的镇痛机制有本质的区别 ,对热痛可能通过抑制PGs合成 ,对炎性痛则可能与NO、EAA有关
Objective To study the analgesic effect of NSAIDs on spinal cord and the relationship with PGs, NO and EAA. Methods Asp, Ind, KT and KPF were selected and the hot water tailing method, formalin test and acetic acid writhing method were used. Results In the heat pain model, 10 ~ 10 ngPGE1 can significantly reduce the pain threshold in mice. Formalin test, 1μg PGE1 significantly inhibited the phase Ⅰ, Ⅱ reaction, 10ng inhibited its phase Ⅰ response, and 0 1,10ng no effect. 10 0,10 0 0 ngPGE1 can reduce the number of writhing in mice, was analgesic effect, and 0 1,10 ng no significant effect. The thermal pain model, NSAIDs spinal cord and systemic administration of effective dose ratio of 1: 20. Each mouse ith 10,10 0 ngPGE1 can antagonize the analgesic effect of NSAIDs. In formalin and writhing, ithPGE1 can not antagonize its effect. In the writhing test, pretreatment with ithL Glu (1 mg · kg -1) and L Arg (0 2 mg · kg -1) reversed the analgesic effect of NSAIDs. Conclusions At the level of the spinal cord, PGE1 has different effects on the pain control of different types. NSAIDs on the nature of the pain of different analgesic mechanism of essential differences in the thermal pain may inhibit the synthesis of PGs, inflammatory pain may be related to NO, EAA