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目的探讨糖尿病大鼠玻璃体血管内皮生长因子(vascular endothelial growth factor,VEGF)及VEGF受体2(kinase insert domain-containing receptor,KDR)水平变化与糖尿病视网膜病变的关系。方法 84只成年Wistar大鼠随机分为观察组64只和对照组20只。观察组采用腹腔注射链脲佐菌素制作糖尿病模型,对照组腹腔注射生理盐水。2组于造模当天及建模后1、2、4周测量大鼠血糖和体质量,4周后采用ELISA法检测大鼠玻璃体VEGF及KDR水平。结果观察组成功制造糖尿病大鼠模型58只,1个月后存活42只,对照组全部存活;造模成功4周时观察组大鼠体质量((297.43±33.50)g)低于对照组((374.57±43.57)g)(P<0.05);造模成功1、2、4周时观察组血糖水平分别为(27.64±5.83)、(26.36±5.12)、(25.29±5.01)mm/L,对照组分别为(5.14±1.76)、(5.36±1.73)、(5.71±1.86)mm/L,差异均有统计学意义(P<0.01);造模成功4周后观察组玻璃体VEGF及KDR水平分别为(0.288±0.026)、(2.936±0.295)μg/L,对照组分别为(0.231±0.021)、(2.394±0.227)μg/L,差异均有统计学意义(P<0.05)。结论大鼠糖尿病模型玻璃体中VEGF和KDR水平变化与糖尿病致早期视网膜病变有关。
Objective To investigate the relationship between the changes of vascular endothelial growth factor (VEGF) and KDR in diabetic rats with diabetic retinopathy. Methods Eighty-four adult Wistar rats were randomly divided into observation group (n = 64) and control group (n = 20). The observation group was given diabetic model by intraperitoneal injection of streptozotocin and the control group by intraperitoneal injection of saline. The blood glucose and body weight of rats in 2 groups were measured on the day of modeling and 1, 2, 4 weeks after modeling. The levels of VEGF and KDR in vitreous were detected by ELISA after 4 weeks. Results In the observation group, 58 diabetic rats were successfully established, 42 survived after 1 month and all the control group survived. The body weight of the observation group (297.43 ± 33.50) g after 4 weeks’ success was lower than that of the control group (374.57 ± 43.57) g) (P <0.05). The blood glucose levels in observation group were (27.64 ± 5.83), (26.36 ± 5.12) and (25.29 ± 5.01) mm / (5.14 ± 1.76), (5.36 ± 1.73) and (5.71 ± 1.86) mm / L respectively in the control group (P <0.01). After 4 weeks of successful model, the levels of VEGF and KDR (0.288 ± 0.026) and (2.936 ± 0.295) μg / L in the control group and (0.231 ± 0.021) and (2.394 ± 0.227) μg / L in the control group, respectively (all P <0.05). Conclusion The changes of VEGF and KDR in the diabetic model of vitreous may be related to the early diabetic retinopathy.