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在吉林省内采集甜椒炭疽病病样,经分离、纯化、观察,确定Colletotrichum nigrumEll.et.Halst(黑色炭疽病)为主要病原菌,并以此作为接种源,对接种液浓度、接种时期、接种方法、接种后温度、湿度及调查时间、调查标准等进行了一系列研究。最后确定用3×106个/mL的孢子悬浮液,在成熟红果期采用离体果针刺法接种,接种后温度(27±1)℃,遮光保湿3 d,保持相对湿度(RH)为95%~100%,7 d后调查病情指数,进行品种抗病性评价。用此抗性鉴定方法对21份甜椒材料进行了筛选鉴定,5份材料表现抗病,10份表现耐病,6份表现感病。
Colletotrichum nigrumEll.et.Halst (black anthracnose) was selected as the main pathogen in Jilin Province, and the concentration of inoculation solution, inoculation period, Inoculation methods, inoculation temperature, humidity and time of investigation, survey standards, conducted a series of studies. Finally, the spore suspension with 3 × 106 cells / mL was used to inoculate in the ripening red fruit stage. The inoculation temperature (27 ± 1) ℃, opaque moisture 3 d and relative humidity 95 % ~ 100%, 7 days after the investigation of disease index, evaluation of disease resistance. The identification of 21 sweet pepper materials was carried out by using this resistance identification method. Five of the materials showed disease resistance, 10 were disease-resistant and 6 were susceptible.