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目的 :探讨广西肝癌高发区 HBV、HCV单一或重叠感染及复制与原发性肝癌发生的关系。方法 :在肝癌高发区选择符合全国诊断标准的原发性肝癌病人 5 2例作为病例组 ,并选择相同乡镇、生活环境、生活习惯、生活水平、年龄± 5岁、同性别的原籍健康人群作为对照组 ,采用微板核酸杂交 - EL ISA法对研究对象血清中的 HBVDNA、HCVRNA及其 HCVRNA基因亚型I型、 型、 型、 型进行定性检测 ,并对两组的阳性率进行配对 χ2检验分析。结果 :肝癌组和对照组的 HBVDNA、HCVR-NA、HBVDNA+HCVRNA、HBVDNA+HCVRNA I型、HBVDNA+HCVRNA 型、HBVDNA+HCVRNA / 型、HB-VDNA +HCVRNA 型的阳性率分别为 78.8% (4 1 / 5 2 )、34.6 % (1 8/ 5 2 )、30 .8% (1 6 / 5 2 )、1 .9% (1 / 5 2 )、2 5 .0 % (1 3/ 5 2 )、1 .9%(1 / 5 2 )、1 .9% (1 / 5 2 )和 1 5 .4 % (8/ 5 2 )、7.7% (4 / 5 2 )、1 .9(1 / 5 2 )、0 .0 % (0 / 5 2 )、1 .9% (1 / 5 2 )、1 .9% (1 / 5 2 )、0 .0 % (0 / 5 2 ) ,其中HBVDNA、HCVRNA、HBVDNA+HCVRNA 型两组间的阳性率比较差异有统计学意义 (均 P <0 .0 0 5 )。结论 :HBV、HCV、HBV和 HCV的重叠感染及复制 ,尤其是 HBV与 HCV基因亚型 型的重叠感染及复制与广西肝癌高发区的肝癌发生有密切关系 ,其中 HBV?
Objective: To investigate the relationship between single and overlapping infection and replication of HBV and HCV and the occurrence of primary hepatocellular carcinoma in high incidence area of liver cancer in Guangxi. Methods: Fifty-two patients with primary hepatocellular carcinoma that meet the national diagnostic criteria were selected as the case group in the high-risk area of HCC. Healthy people with same-age township, living environment, living habits, In the control group, the HBVDNA, HCVRNA and the HCVRNA subtype I, type, type and type in the sera of the subjects were qualitatively detected by microplate nucleic acid hybridization - ELISA method, and the positive rate of the two groups was tested by χ2 test analysis. RESULTS: The positive rates of HBVDNA, HCVR-NA, HBVDNA + HCVRNA, HBVDNA + HCVRNA I, HBVDNA + HCVRNA, HBVDNA + HCVRNA / HBVDNA and HCVRNA were 78.8% 1/5 2), 34.6% (18/52), 30.8% (16/52), 1.9% (1/52), 25.5% (1/5 2) ), 1.9% (1/5 2), 1.9% (1/5 2), and 1.54% (8/5 2), 7.7% (4/5 2), 1 .9 / 5 2), 0 .0% (0/5 2), 1 .9% (1/5 2), 1 .9% (1/5 2), 0 .0% (0/5 2) The positive rates of HBVDNA, HCVRNA, HBVDNA + HCVRNA between the two groups were significantly different (all P <0.05). Conclusion: The overlapping infection and replication of HBV, HCV, HBV and HCV, especially the overlapping infection and replication of HBV and HCV subtypes are closely related to the occurrence of hepatocellular carcinoma in Guangxi.