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[目的]探讨黑果小檗醇提物对小鼠肝损伤模型的影响,从而为黑果小檗对肝损伤疾病提供现代医学研究佐证。[方法]利用四氯化碳(CCl4)化学法建立小鼠肝损伤模型,并用黑果小檗醇提物进行干预,以观察其对肝功能指标的影响。将20只小鼠随机分为低剂量组、中剂量组、高剂量组、空白对照组和模型对照组,分别腹腔注射黑果小檗醇提物0.1、0.2、0.3 g/(10 g·BW),空白对照组同剂量腹腔注射生理盐水,模型对照组同剂量腹腔注射四氯化碳,在试验初期,第0天除空白对照组外,其他各组均腹腔注射5%CCl_4 0.1 m L/(10 g·BW)1次,黑果小檗醇提物连续给药7 d。第8天采血检测生化指标及脏器系数。[结果]黑果小檗高剂量组与模型对照组比较有极显著差异(P<0.01),黑果小檗低剂量组和中剂量组与模型对照组比较AST、ALT活性降低,但无显著差异(P>0.05);模型对照组肝脏系数增加,肝脏与脾脏系数各组之间有差异性;高剂量组肝脏系数与模型对照组比较,差异显著(P<0.05)。[结论]高浓度黑果小檗对四氯化碳引起的肝损伤具有保护作用。
[Objective] The research aimed to discuss the effect of ethanol extract of Berberis on mice model of liver injury, and provide evidence for modern medical research on the disease of liver injury caused by barberry. [Method] The model of hepatic injury in mice was established by using CCl4 chemistry method, and the intervention of black berry alcohol extract was used to observe its effect on liver function indexes. Twenty mice were randomly divided into low dose group, middle dose group, high dose group, blank control group and model control group. The mice were injected intraperitoneally with 0.1, 0.2, 0.3 g / (10 g · BW ). The blank control group was given the same dose of intraperitoneal injection of normal saline, and the model control group was intraperitoneally injected with carbon tetrachloride. At the beginning of the experiment, on the 0th day except for the blank control group, the other groups were intraperitoneally injected with 5% CCl 4 0.1 m L / (10 g · BW) 1 times, black berry alcohol extract continuous administration of 7 d. On the 8th day, biochemical indexes and viscera coefficient of blood sampling were measured. [Result] Compared with the model control group, there was a significant difference (P <0.01) in the high-dose Berberis group and the model control group, while the activities of AST and ALT in the low-dose Berberis group and the middle-dose group were lower than those in the model control group (P> 0.05). There was a significant difference between the model control group and the model control group (P <0.05). There was significant difference between the model group and the model control group (P <0.05). [Conclusion] Barberry with high concentration had a protective effect on carbon tetrachloride-induced liver injury.