论文部分内容阅读
目的探讨性别分化关键期三丁基氯化锡(TBTC)暴露对大鼠性成熟后睾丸酶活力和生精功能的影响。方法将16只健康成年SPF级妊娠Wistar大鼠随机分为高(5.0mg/kg)、中(2.5mg/kg)、低剂量(1.0mg/kg)TBTC染毒组和溶剂对照组(玉米油),每组4只。从妊娠第12天起,采用经口灌胃方式进行染毒,每天1次,直至妊娠第20天,灌胃剂量为5.0ml/kg。出生后第70天,每组随机抽取10只雄性仔鼠,称重后处死,分离双侧睾丸及附睾组织,测定附睾精子数及睾丸酶[乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、酸性磷酸酶(ACP)、一氧化氮合酶(NOS)]活力。结果与溶剂对照组比较,各TBTC染毒组孕鼠妊娠第12天及第20天的体重和体重增长量差异无统计学意义(P>0.05)。与溶剂对照组比较,高、中剂量TBTC染毒组雄性仔鼠断乳后体重增长较慢,差异均有统计学意义(P<0.01)。TBTC染毒组附睾尾精子数随TBTC染毒剂量的升高呈上升趋势,差异有统计学意义(P<0.01)。与溶剂对照组相比,高、中、低剂量TBTC染毒组雄性仔鼠LDH活力较高,高、中剂量TBTC染毒组雄性仔鼠SDH活力较高,差异均有统计学意义(P<0.01)。各TBTC染毒组雄性仔鼠ACP和NOS活力间比较,差异均无统计学意义(P>0.05)。未发现睾丸组织结构发生改变。结论性别分化关键期TBTC染毒对雄性仔鼠具有内分泌干扰作用,减缓了雄性仔鼠的体重增长,促进了其精子发生和LDH、SDH的活力。
Objective To investigate the effect of tributyltin chloride (TBTC) exposure on the activity of testis and the function of spermatogenesis in the sexual maturation stage of rats. Methods Twenty-six healthy adult Wistar rats of SPF grade were randomly divided into high (5.0mg / kg), middle (2.5mg / kg), low dose (1.0mg / kg) TBTC exposure groups and solvent control group ), 4 in each group. From the 12th day of gestation, the oral gavage method was used to take the medicine once daily until the 20th day of gestation, and the intragastric dose was 5.0ml / kg. On the 70th day after birth, 10 male offspring were randomly selected from each group and weighed and sacrificed. The testis and epididymis were separated from the testis and epididymis to determine the number of spermatozoa and testicular enzymes (lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), acid phosphatase (ACP), nitric oxide synthase (NOS)] activity. Results Compared with the solvent control group, there was no significant difference in body weight and body weight gain on the 12th and 20th days of gestation in each TBTC exposure group (P> 0.05). Compared with the solvent control group, the weight gain of male and female TBTC poisoning rats after weaning were slower than that of the solvent control group, the differences were statistically significant (P <0.01). The number of sperm epididymis in the TBTC group increased with the dose of TBTC, the difference was statistically significant (P <0.01). Compared with the solvent control group, the LDH activity of male offspring in high, medium and low doses of TBTC was higher than that in the solvent control group, and the SDH activity of male offspring of high and medium dose TBTC was higher than that of the solvent control group (P < 0.01). There was no significant difference in ACP and NOS activity among male offspring of TBTC exposure groups (P> 0.05). Did not find the testicular tissue structure changes. Conclusion TBTC exposure at the critical stage of sex differentiation has the effect of endocrine disruption on male offspring, slowing the weight gain of male offspring, and promoting the spermatogenesis and the viability of LDH and SDH.