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目的探讨在体外培养液中添加LH(促黄体生成激素)对人未成熟卵母细胞体外培养成熟(IVM)及继续发育潜能的影响。方法 56例多囊卵巢综合征(PCOS)患者进行64个IVM周期治疗,其中36例共36周期在体外培养液中添加LH;另20例共28周期培养液中未添加LH。结果 LH组和对照组的卵母细胞体外培养成熟率、受精率、卵裂率、优胚率、平均胚胎移植数分别为:65.47%vs 73.02%、70.91%vs 78.26%、92.31%vs 86.11%、48.15%vs45.98%、2.07vs 2.00,差异均无统计学意义(P>0.05);胚胎种植率、临床妊娠率分别为:20%vs8.33%、41.67%vs 28.57%,差异有统计学意义(P<0.05)。结论 LH可能改善了未成熟卵母细胞体外成熟后胚胎的继续发育潜能。
Objective To investigate the effect of LH (luteinising hormone) supplementation on in vitro culture of human immature oocytes in vitro (IVM) and its developmental potential. Methods Sixty-six patients with PCOS were treated with 64 cycles of IVM, of which 36 cases were given LH in vitro in 36 cycles and LH was not added in the other 20 cases with 28 cycles. Results The maturation rate, fertilization rate, cleavage rate, excellent embryo rate and mean number of embryo transfer of oocytes cultured in LH group and control group were 65.47% vs 73.02%, 70.91% vs 78.26%, 92.31% vs 86.11% , 48.15% vs45.98%, 2.07vs 2.00, respectively (P> 0.05). The rate of embryo implantation and clinical pregnancy were 20% vs 8.33%, 41.67% vs 28.57%, respectively Significance (P <0.05). Conclusion LH may improve the immature oocyte maturation embryos in vitro development potential.