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目的观察腺苷A1受体激动剂对脊髓缺血性损伤后的神经元凋亡的影响。方法建立兔脊髓缺血模型,给予腺苷A1受体激动剂(CPA),观察兔神经功能恢复,通过病理切片,计算脊髓前角正常神经元,再应用免疫荧光染色检测BCL-2表达水平,western-blot的方法观察caspase3、caspase9、BCL-2、BCL-X1、BAX、BAD表达水平。结果与DMSO组相比,CPA组兔神经功能明显改善。caspase3、caspase9、BCL-2、BCL-X1表达水平显著高于DMSO组,而BAX、BAD表达水平则明显降低。结论腺苷A1受体激动剂可能通过抑制神经元凋亡对脊髓缺血损伤发挥保护作用。
Objective To investigate the effect of adenosine A1 receptor agonist on neuronal apoptosis after spinal cord ischemic injury. Methods The model of spinal cord ischemia in rabbits was established. Adenosine A1 receptor agonist (CPA) was given to observe the recovery of neural function in rabbits. Normal neurons in anterior horn of spinal cord were counted by pathological sections. The expression of BCL-2 was detected by immunofluorescence staining. western-blot method to observe the expression of caspase3, caspase9, BCL-2, BCL-X1, BAX, BAD. Results Compared with DMSO group, the neurological function of rabbits in CPA group was significantly improved. The expression levels of caspase3, caspase9, BCL-2 and BCL-X1 were significantly higher than those in DMSO group, while the expressions of BAX and BAD were significantly decreased. Conclusion Adenosine A1 receptor agonists may play a protective role in ischemic injury of the spinal cord by inhibiting neuronal apoptosis.