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采用改良的ER培养基,附加不同种类的植物生长调节物质,使茶树接近成熟的种子的子叶基部及子叶柄形成胚状体。详细观察了胚状体形成过程中形态学上和细胞组织学上的变化。初步肯定了胚状体起源于子叶表皮细胞;胚状体在胚胎发生的最初分隔方式上与合子胚不同,发育过程与合子胚较一致。进一步观察到不同种类的植物生长调节物质的用量及配合用量影响胚状体的分化及小植株的形成。并详细介绍了茶树组培苗移栽成活的方法。
Using modified ER medium supplemented with different kinds of plant growth regulators, the cotyledons of the cotyledons and cotyledons of the tea tree near the mature seed form embryoid bodies. Morphological and histological changes in embryoid body formation were observed in detail. Preliminary identification of embryoid bodies originated in the cotyledon epidermal cells; embryoid bodies in the initial separation of embryonic and zygotic embryos different development process and zygotic embryo more consistent. It was further observed that the amount and amount of different kinds of plant growth regulating substances affected embryoid body differentiation and plantlet formation. And introduced in detail the method of transplanting survival of tea tree tissue culture seedlings.