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目的: 探讨survivin在活化T细胞的表达及其意义。方法: 经丝裂原与同种抗原刺激激活T细胞, 免疫细胞化学染色后观察survivin和增殖细胞核抗原(PCNA)的表达。用流式细胞术检测CD3、CD25、Survivin的表达及细胞凋亡。给BALB/c裸鼠输注C57BL/6小鼠脾细胞后第 4~7天, 观察肝脏内T细胞浸润和survivin的表达。结果: ConA刺激后培养的脾细胞可表达survivin(表达不局限于G2 /M期 )。survivin+细胞为T细胞, T淋巴母细胞可同时表达CD25和survivin。survivin+细胞中仅部分细胞表达CD25。ConA刺激淋巴细胞后第 6天, 凋亡细胞的百分率明显增加 ( 22. 90% vs5. 23%P<0. 001)。于BALB/c裸鼠输注C57BL/6小鼠的脾细胞后,于第 4~7天, 在BALB/c裸鼠肝脏中可观察到T细胞浸润并表达Survivin。结论: T细胞激活后可表达survivin, 故可作为活化T细胞的标志。T细胞持续表达survivin, 需要在体内与抗原持续接触。
Objective: To investigate the expression of survivin in activated T cells and its significance. Methods: T cells were stimulated by mitogen and alloantigen. The expression of survivin and proliferating cell nuclear antigen (PCNA) were observed by immunocytochemistry. Flow cytometry was used to detect the expression of CD3, CD25, Survivin and apoptosis. BALB / c nude mice were infused with C57BL / 6 mice splenocytes after 4 to 7 days to observe the liver T cell infiltration and survivin expression. Results: The splenocytes cultured with ConA stimulated the expression of survivin (the expression was not limited to G2 / M phase). Survivin + cells are T cells and T lymphoblasts express both CD25 and survivin. Only a few cells in the survivin + cells express CD25. On the 6th day after ConA stimulated lymphocytes, the percentage of apoptotic cells increased significantly (22.90% vs5.23% P <0.001). After BALB / c nude mice were infused with splenocytes of C57BL / 6 mice, T cell infiltration and Survivin expression were observed in BALB / c nude mice livers on the 4th to 7th day. Conclusion: T-cell activation can express survivin, which can be used as a marker of activated T cells. T cells continue to express survivin, requiring constant contact with the antigen in vivo.