人类成熟卵母细胞是体内最大的细胞,直径约120μm左右。与精子和植入前4~8细胞阶段胚胎相比,冻融过程对卵母细胞更容易造成损伤。自1948年利用甘油作冷冻保护剂冻存牛精子获得成功后,直至1972年冻融小鼠胚胎才获子代出生。1983年,人类第一例冻融胚胎“试管婴儿”诞生。迄今在辅 Human mature oocytes are the largest cells in the body, about 120μm in diameter. The freeze-thaw process is more likely to cause damage to oocytes than spermatozoa and embryos 4 to 8 prior to implantation. Since the successful cryopreservation of bovine sperm with glycerol as a cryoprotectant in 1948, the embryo was frozen and thawed until its birth in 1972. In 1983, the first case of human embryo frozen embryo “test tube baby” was born. So far in the auxiliary