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小动物高频超声技术是目前先进的实时无创分析技术,在基础研究和临床应用领域具有重要作用.详细介绍了高频超声技术仪器的背景及分类、技术特点、超声成像范围和软件,并对高频超声技术在基础研究和临床领域的应用情况进行总结,为其他研究者了解和使用高频超声技术进行科研工作提供借鉴.“,”High-frequency ultrasound technology for small animal is an advanced real-time non-invasive analysis tool, which plays an important role in many basic research fields and clinic application. This paper introduces the background, classification, technical characteristics, ultrasonic imaging range and software of high-frequency ultrasound technology instruments in detail, and summarizes the application of high-frequency ultrasound technology in different research fields, so as to provide reference for other researchers to understand and use high-frequency ultrasound technology for scientific research.