论文部分内容阅读
目的建立小鼠Ⅱ度烧伤模型,观察苦地湿敷剂(KDSFJ)的治疗作用。方法采用80℃恒温水烫小鼠皮肤3s,制作60只Ⅱ度烧伤小鼠模型,造模成功后随机将其分成A,B,C三组,A组用KDSFJ,B组用硼酸外喷伤口,均为2次/d,C组不做任何处理。每天下午用药前肉眼观察皮损变化,并记录创面结痂、脱痂和愈合时间,于第1,4,8,12和19天时用Nagelschmidt法测量创面或疤痕面积。结果重复测量方差分析表明,3种方法对鼠烧伤面积的影响趋势不相同,A组皮损面积呈直线下降趋势,B和C组在治疗第4天时皮损面积达到顶峰,后面积不断下降,组间差异有统计学意义(P=0.000),三组间差异也均有统计学意义(P<0.03)。造模后第19天,三组小鼠皮损疤痕面积比较,A组疤痕面积最小,差异有统计学意义(P=0.008)。A组愈合时间最短,第12天时,所有病例均愈合。结论 KDSFJ能快速控制烧伤导致的炎症反应,控制炎症扩散,减少愈合时疤痕的形成,缩短愈合时间,值得进一步深入研究。
Objective To establish the second degree burn model in mice and observe the therapeutic effect of KDSFJ. Methods The mouse skin was treated with water at 80 ℃ for 3 seconds to make 60 second-degree burn mice. After successful modeling, the mice were randomly divided into three groups: A, B and C, KDSFJ in group A, , Both were 2 times / d, C group did not do any treatment. Every afternoon afternoon, the changes of the lesions were observed with the naked eye before the treatment, and the wounds’ scabs, scabs and healing time were recorded. On the 1st, 4th, 8th, 12th and 19th days, the wound or scar area was measured by Nagelschmidt method. Results Repeated measures ANOVA showed that the three methods had different trends on the burn area of rats. The lesions in group A showed a straight downward trend. The lesions in group B and C reached the peak on the fourth day after treatment, The differences between the two groups were statistically significant (P = 0.000), and the differences among the three groups were also statistically significant (P <0.03). On the 19th day after modeling, the scar area of the three groups was the smallest, the difference was statistically significant (P = 0.008). Group A had the shortest healing time, and on the 12th day, all the cases healed. Conclusion KDSFJ can rapidly control the inflammatory reaction induced by burns, control the spread of inflammation, reduce the formation of scar during healing and shorten the healing time, which deserves further study.