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目的了解副溶血性弧菌致病力及药敏等情况,以在副溶血性弧菌中毒机理及临床治疗方面提供科学依据。方法按国家标准GB/T-4789-2008[1]操作规程进行副溶血性弧菌致病因素检测。结果60株副溶血性弧菌(病人分离的菌株37株,外环境菌株23株),刚果红侵袭力试验均阴性;内毒素检测96.7%阳性;溶血毒素检测病人分离的菌株100%阳性,外环境分离的菌株均阴性。产毒荧光定量PCR体外检测副溶血弧菌,病人分离的菌株97.3%(36/37)阳性,外环境分离的菌株13.0%(3/23);对治疗由本菌引起中毒病人,复方新诺明、氯霉素、庆大霉素等是首选药物。结论副溶血性弧菌致病性与其产生的内毒素、毒力、溶血毒素有密切相关。
Objective To understand the virulence of Vibrio parahaemolyticus and its susceptibility to Vibrio parahaemolyticus to provide a scientific basis for its mechanism and clinical treatment. Methods According to the national standard GB / T-4789-2008 [1] operating procedures Vibrio parahaemolyticus pathogenic factors detection. Results 60 strains of Vibrio parahaemolyticus (37 isolated strains and 23 strains of external environmental strains) and Congo red invasiveness test were negative. Endotoxin was 96.7% positive. The hemolytic toxin isolated strains were 100% Environmental isolates were negative. Virulence quantitative PCR was used to detect Vibrio parahaemolyticus in vitro. 97.3% (36/37) isolates were positive in patients and 13.0% (3/23) were isolates in external environment. , Chloramphenicol, gentamicin is the drug of choice. Conclusion The pathogenicity of Vibrio parahaemolyticus is closely related to the endotoxin, virulence and hemolytic toxin produced by it.