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目的:探讨瘦素对人脐静脉内皮细胞(ECV-304)表达白细胞介素-8(IL-8)的影响。方法:用不同浓度的瘦素刺激ECV-304,检测ECV-304表达IL-8的情况。结果:在相同作用时间下,随着瘦素浓度的升高,IL-8蛋白及IL-8的mRNA的表达也随之升高;在相同瘦素浓度下,随着作用时间的延长,IL-8蛋白及IL-8的mRNA的表达也随之升高。结论:瘦素可以刺激ECV-304表达IL-8,而且呈时间和剂量相关性。
Objective: To investigate the effect of leptin on the expression of interleukin-8 (IL-8) in human umbilical vein endothelial cells (ECV-304). Methods: ECV-304 was stimulated with different concentrations of leptin to detect the expression of IL-8 in ECV-304. Results: Under the same action time, the expression of IL-8 protein and IL-8 mRNA increased with the increase of leptin concentration. Under the same leptin concentration, IL -8 protein and IL-8 mRNA expression also increased. Conclusion: Leptin can stimulate the expression of IL-8 in ECV-304 cells in a time-and dose-dependent manner.