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以紫背菜茎段为外植体,通过不定芽诱导及生根培养,获得了再生植株;以无菌苗根茎叶为外植体,研究愈伤组织的诱导与分化。结果表明:茎段诱导不定芽培养基以MS+6-BA1.0mg/L+NAA0.2mg/L+3.0%蔗糖为佳。试管苗生根培养在1/2MS添加NAA浓度0～1.2mg/L范围内均可,但NAA浓度增加时,诱导生根时间可缩短。3种外植体均可成功诱导愈伤组织,以根外植体诱导率最高,培养基为MS+6-BA0.3mg/L+2,4-D1.0mg/L+4.5%蔗糖。愈伤组织分化培养仅得到不定根。
Regeneration plants were obtained by adventitious bud induction and rooting. The roots, stems and leaves of sterile plants were used as explants to study the induction and differentiation of callus. The results showed that MS + 6-BA1.0mg / L + NAA0.2mg / L + 3.0% sucrose was the best medium for adventitious shoot induction in stem segments. The rooting of test-tube plantlets could be done within 1 / 2MS with NAA concentration of 0-1.2mg / L. However, when NAA concentration was increased, rooting time could be shortened. The explants of all the three explants could successfully induce callus, and the highest induction rate of root explants was MS + 6-BA0.3mg / L + 2,4-D1.0mg / L + 4.5% sucrose. Callus differentiation culture only obtained adventitious roots.