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选择0#柴油和平湖原油乳化液对缢蛏(Sinonovacula constricta)进行氧化胁迫实验,选取典型的抗氧化酶-超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷光甘肽硫转移酶(GST)及过氧化物酶(POD)用于衡量油类污染物对生物体造成的氧化压力大小.此外,结合综合生物标志物响应(Integrated Biomarker Responses,IBR)指标,对2种石油污染物对缢蛏的毒性响应进行定量化评价.结果表明,不同浓度的0#柴油和平湖原油对缢蛏消化腺中的4种酶表现出不同程度的诱导效应,各试验组在暴露前期均表现出诱导或抑制,但对4种酶的影响存在时间顺序性,SOD、CAT和GST的酶活性表现为升高-降低的过程,POD表现为降低-升高的过程,活性达到峰值的时间SOD和CAT要早于GST和POD.结合计算出的IBR数值来看,高浓度0#柴油能够引起最为显著的生物效应变化,显示该石油污染物高毒性的特征,0#柴油生物毒性大于平湖原油生物毒性.
Oxidative stress experiments on Sinonovacula constricta were carried out using 0 # diesel oil and Pinghu crude oil emulsion. Typical antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and peroxidase (POD) were used to measure the oxidative stress caused by oil pollutants to living organisms.In addition, combined with IBM (Integrated Biomarker Responses) indicators, two petroleum pollutants The results showed that different concentrations of 0 # diesel oil and Pinghu crude oil had different degrees of induction effects on four enzymes in the digestive gland of Zucchini, and all the experimental groups showed induction or But there was a chronological effect on the four enzymes. The enzyme activities of SOD, CAT and GST showed a process of increase-decrease, POD showed a process of decrease-increase, the time of peak of activity was SOD and CAT As early as GST and POD. Combined with the calculated IBR values, the high concentration of 0 # diesel oil can cause the most significant biological effects, showing the characteristics of the high toxicity of petroleum pollutants, 0 # diesel biological toxicity greater than Pinghu crude oil organisms Sex.