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合成与结构表征了 4个钴卟啉配合物 Co(Por) ,Por=5 ,10 ,15 ,2 0 -四 - [(3,4,5 -三甲氧基苯基 )卟啉(1) ,(对甲基苯基 )卟啉 (2 ) ,(对氯苯基 )卟啉 (3) ,(对磺酸基苯基 )卟啉 (4) ].并用它们作为超氧化物歧化酶 (SOD)和过氧化氢酶 (CAT)模型物进行了双功能模拟酶活性的测试 :核黄素 -蛋氨酸光照法证实 ,在 10 - 5～ 10 - 6 mol/ L 浓度范围内 4种配合物均有消除超氧自由基 (O-·2 )的作用 ;分光光度法证实具有催化 H2 O2 的分解作用 ,其分解率随浓度的增大而增大 ;用小鼠肝匀浆法测定 ,四种钴卟啉均表明有良好的抗脂质过氧化作用 .
Synthesis and structure of four porphyrin complexes Co (Por), Por = 5, 10, 15 and 20 - tetrakis [3,4,5 - trimethoxyphenyl) porphyrin (P-methylphenyl) porphyrin (2), (p-chlorophenyl) porphyrin (3), and p-sulfophenylalanyl porphyrin (4)] were used as superoxide dismutase ) And catalase (CAT) were used to test the activity of bifunctional mimic enzyme. The results of photometric analysis with riboflavin - methionine showed that all the four complexes in the range of 10 - 5 ~ 10 - 6 mol / L (O · 2) was eliminated by spectrophotometry. The decomposition of H 2 O 2 was confirmed by spectrophotometry, the decomposition rate of H 2 O 2 increased with the increase of concentration of H 2 O 2. Porphyrin both showed good anti-lipid peroxidation.