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通过HPLC确定灌胃芦荟药物在家兔血清中达到峰值时相,确定最佳采血时间,制备芦荟药物血清,将血清加入糖基化终产物(AGEs)作用过的肾系膜细胞(RGMC)中,孵育24 h。之后,检测细胞内谷胱甘肽(GSH)和氨基葡萄糖苷酶(NAG)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、山梨醇脱氢酶(SDH)和醛糖还原酶(AR)的活性,研究芦荟药物血清对RGMC的保护效果,初步探讨芦荟防治糖尿病的机理。结果表明,芦荟药物在家兔灌胃后2 h的血清中血液达到相对峰值;AGEs作用过的细胞经芦荟药物血清处理后,其GSH含量、SOD活性均较未加芦荟处理的A组有所升高,且差异极显著(P<0.01),其SDH和AR的活性也较未经芦荟处理的A组极显著下降(P<0.01)。表明芦荟药物血清对AGEs损伤后的RGMC细胞有一定的保护修复作用,芦荟保护RGMC细胞的机制可能是通过改善细胞的氧化应激状态,同时降低多元醇通路的2种关键酶的活性而起作用的。
Determination of the peak phase in serum of rabbits by gavage with Aloe vera drug, determination of the best blood sampling time, preparation of Aloe drug serum, and addition of serum to AGEs-induced mesangial cells (RGMC) , Incubate for 24 h. Afterwards, intracellular glutathione (GSH) and aminoglucosidase (NAG) levels, superoxide dismutase (SOD), catalase (CAT), sorbitol dehydrogenase (SDH), and aldose were measured. The activity of reductase (AR) was studied to study the protective effect of aloe vera drug serum on RGMC, and the mechanism of aloe to prevent and treat diabetes was preliminarily explored. The results showed that the serum in the serum of Aloe vera drug reached a relative peak at 2 h after instillation in rabbits; the GSH content and SOD activity of Aloe vera drug-treated cells after Aloe drug serum treatment were higher than those of A group without Aloe vera treatment. The difference was extremely significant (P<0.01), and the activity of SDH and AR was also significantly lower than that of group A without aloe treatment (P<0.01). It shows that Aloe drug serum has a protective and repairing effect on RGMC cells after AGEs injury. The mechanism of aloe protecting RGMC cells may be through improving the oxidative stress state of cells and reducing the activity of two key enzymes of the polyol pathway. of.