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目的研究铜绿假单胞菌临床分离株的遗传基因型,明确铜绿假单胞菌携带株的流行特征。方法用多位点序列分型技术(MLST),以铜绿假单胞菌7个管家基因acsA、aroE、guaA、mutL、nuoD、ppsA和trpE的内部特定核酸片段作为检测的靶基因,对60株铜绿假单胞菌的靶基因进行PCR扩增和测序,通过eBURST方法对其进行进化聚类分析,同时通过SplitsTree软件进行菌株基因遗传进化分析。结果 MLST分析显示60株铜绿假单胞菌可分为11个ST型,即ST262、ST195、ST767、ST274、ST527、ST849、ST639、ST871、ST244、ST636和ST645型,未发现新的型别;其中ST244和ST274是优势ST型别,分别占26.67%,18.33%;且ST244型在ICU及呼吸内科数量显著高于其他科室(P<0.05)。结论本地区以ST244和ST274为优势克隆株,尤其在ICU及呼吸内科数量显著高于其他科室,提示医院内存在小范围的传播;MLST对于流行病学研究具有重要意义。
Objective To study the genotypes of clinical isolates of Pseudomonas aeruginosa and clarify the epidemiological characteristics of Pseudomonas aeruginosa carriers. Methods Multi-site sequence typing (MLST) was used to detect the specific target genes of seven housekeeping genes acsA, aroE, guaA, mutL, nuoD, ppsA and trpE of Pseudomonas aeruginosa, The target gene of Pseudomonas aeruginosa was amplified by PCR and sequenced. The eBURST method was used to carry out phylogenetic analysis of the target gene, and phylogenetic analysis was carried out by SplitsTree software. Results MLST analysis showed that 60 strains of Pseudomonas aeruginosa could be divided into 11 ST types, namely, ST262, ST195, ST767, ST274, ST527, ST849, ST639, ST871, ST244, ST636 and ST645, Among them, ST244 and ST274 were the predominant type of ST, accounting for 26.67% and 18.33% respectively; and the number of ST244 in ICU and respiratory medicine was significantly higher than that in other departments (P <0.05). Conclusion ST244 and ST274 are predominant clones in this area, especially in the ICU and respiratory medicine. The number of ICU and respiratory medicine is significantly higher than that of other departments, suggesting a small-scale spread within the hospital. MLST is of great significance for epidemiological studies.