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通过测定二氧六环稀释的油性注射剂的椭圆度(Ellipticity)可直接定量注射剂书的睾酮苯丙酸酯、诺龙苯丙酸酯和黄体酮等活性成分。在测定Δ~(?)-3-酮甾体圆二色谱(CD)和CD 差谱时,环已烷为最适溶剂。但作者实验表明,油注射剂中的保护剂苯甲醇(10%v/v)可使以环己烷为稀释溶剂的睾酮苯丙酸酯的CD 谱变形。以320～350 nm 区段中最大负椭圆度处的椭圆度值之比(R)对苯甲醇浓度作图,可见以环己烷为溶剂时,随苯甲醇浓度增加,R 明显上升;而以二氧六环为溶剂时,苯甲醇浓度对R 几无影响。油注射剂中常用的葵花子油于330nm 处有最大负椭圆度,本测定用342.8nm 波长,此时葵花子油无圆二色性。测定方法:0.5ml 注射剂以二氧六环(KOH,金
The active ingredients such as testosterone, norebprone and progesterone can be directly quantified by measuring the ellipticity of dioxane-diluted oily injection. In the determination of Δ ~ (?) - 3-ketosteroid circular dichroism (CD) and CD differential spectra, cyclohexane as the optimum solvent. However, experiments by the authors showed that benzyl alcohol (10% v / v), a preservative in oil injections, can deform the CD spectrum of testosterone phenylpropionate in cyclohexane as a diluting solvent. The ratio of the degree of ovality (R) at the maximum negative ovality in the range of 320-350 nm was plotted against the concentration of benzyl alcohol. It can be seen that when cyclohexane was used as the solvent, the R increased significantly with increasing concentration of benzyl alcohol. Dioxane as a solvent, benzyl alcohol concentration on the R few no effect. The sunflower oil commonly used in oil injection has the largest negative ovality at 330 nm. The wavelength of 342.8 nm is used in this determination. At this time, the sunflower oil has no circular dichroism. Determination: 0.5ml injection to dioxane (KOH, gold