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目的观察皮肤再生医疗技术(MEBT/MEBO)对大鼠慢性难愈合皮肤创面组织匀浆中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)水平的影响。方法将54只慢性难愈合创面模型SD大鼠随机分为观察组、贝复济组、模型组各18只,造模成功后以湿润暴露疗法(MEBT)创面分别贴两层湿润烧伤膏(MEBO)纱条、贝复济浸透纱布、生理盐水纱布;空白组建立全层皮肤缺损模型,不加任何干预措施。造模后第3、7、14天各组随机处死5只大鼠,取相同部位皮肤溃疡创面肉芽组织,采用HE染色观察创面组织病理,ELISA法检测创面组织匀浆中的TNF-α、IL-6水平。结果造模后第14天,观察组、贝复济组大鼠创面组织中大量微血管生成,炎症细胞消失,创面恢复良好。各组大鼠创面组织匀浆中TNF-α、IL-6水平随时间延长均呈下降趋势,但以观察组下降最明显,模型组下降最缓慢;观察组、贝复济组创面组织匀浆中TNF-α、IL-6水平始终高于空白组(P均<0.01);且造模后第7天,观察组创面组织匀浆中TNF-α、IL-6水平低于贝复济组(P均<0.01);造模后第14天,观察组与贝复济组创面组织匀浆中TNF-α、IL-6水平比较无统计学意义(P均﹥0.05)。结论 MEBT/MEBO可以合理控制炎症因子TNF-α、IL-6的释放,改变病理炎症反应状态,从而促进创面愈合。
Objective To observe the effect of MEBT / MEBO on the levels of TNF-α and IL-6 in the wounds of chronic non-healing skin wounds in rats. Methods Totally 54 SD rats with chronic refractory wounds were randomly divided into observation group, Beifuji group and model group, with 18 MEBT wounds treated with MEBO (MEBO) respectively ) Gauze, shellfish dipping gauze, saline gauze; blank group to establish a full-thickness skin defect model, without any intervention. On the 3rd, 7th and 14th day after operation, 5 rats were randomly sacrificed in each group, and the granulation tissue of wounds on the same site were taken. The pathological changes of wounds were observed by HE staining. The levels of TNF-α, IL- -6 level. Results On the 14th day after the model was established, a large amount of microvessel was formed in the wound tissues of the observation group and the Beifuji group, the inflammatory cells disappeared and the wound recovered well. The levels of TNF-α and IL-6 in wound tissue homogenate of rats in each group decreased with time, but the decrease was the most obvious in the observation group and the slowest in the model group. In the observation group and the Beifuji group, TNF-α and IL-6 levels in the wound tissue in the observation group were lower than those in the Beifuji group (P <0.01) (All P <0.01). On the 14th day after modeling, there was no significant difference in TNF-α and IL-6 levels between the observation group and Beifuji group (P> 0.05). Conclusion MEBT / MEBO can reasonably control the release of inflammatory cytokines TNF-α and IL-6 and change the pathological inflammatory response so as to promote wound healing.