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利用~１Ｈ ＮＭＲ谱研究枯草杆菌中性蛋白酶与无机金属化合物（ＣｏＣｌ_２， ＮｉＣｌ_２）间的相互作用．结果发现，原酶活性中心金属离子Ｚｎ（Ⅱ）可以与外加ＣｏＣｌ_２，ＮｉＣｌ_２发生直接相互作用，被Ｃｏ（Ⅱ），Ｎｉ（Ⅱ）部分置换而生成了酶的Ｃｏ（Ⅱ），Ｎｉ（Ⅱ）取代衍生物；获得了该酶的两种金属取代衍生物的~１ＨＮＭＲ谱图，同时论证了该酶活性中心的配位结构．
Study on the Interaction between Bacillus subtilis Neutral Protease and Inorganic Metal Compounds (CoCl_2, NiCl_2) by ~ 1H NMR Spectroscopy. The results showed that Zn (Ⅱ) could directly interact with CoCl_2 and NiCl_2, and Co (Ⅱ) and Ni (Ⅱ) replaced Co (Ⅱ) and Ni Substituted derivatives; ~ 1HNMR spectra of two metal-substituted derivatives of the enzyme were obtained, and the coordination structure of the active center of the enzyme was demonstrated.