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目的:采用HPLC建立两面针药材特征指纹图的分析方法,为市场上流通的两面针药材提供质控参考。方法:建立两面针HPLC特征指纹图谱方法,并结合相似度评价及系统聚类分析方法对市场上购买的25批样品药材进行初步的鉴别分析,鉴定两面针药材真伪及品质优劣。结果:在25批市场抽样的药材中,仅有8批被鉴定为质量较好的正品,有7批被鉴定为次品,7批被鉴定为两面针常见伪品飞龙掌血,3批被鉴定为伪品。结论:本法准确、方便、可靠,可用于两面针药材的鉴别和质量控制。
OBJECTIVE: To establish a method for the analysis of fingerprints of medicinal materials of two-sided needles by HPLC, and to provide quality control reference for two-sided medicinal materials circulating in the market. Methods: The HPLC fingerprints of two-sided needles were established. Based on similarity evaluation and cluster analysis, twenty-five batches of medicinal herbs purchased from the market were initially identified and analyzed to verify the authenticity and quality of the two-stitched herbs. Results: Of the 25 batches of medicinal herbs sampled from the market, only 8 batches were identified as genuine products with good quality, 7 batches were identified as defective products, 7 batches were identified as two-sided needle common fake dragon claw blood, 3 batches were Identified as false. Conclusion: This method is accurate, convenient and reliable, and can be used for identification and quality control of two-sided needles.