论文部分内容阅读
利用RAPD对我国东北地区引致小麦赤霉病的2种镰刀菌进行种群分析,并与江苏和西北的菌株进行了比较。共筛选出16个引物用于扩增反应,其扩增图谱显示供试菌株在种间和种内均具多态性。多数引物扩增出了种的特征性谱带,可用作种的鉴定。综合所有谱带系统聚类所得树状图明显地将Fusarium graminearum的34个菌株和F.avenaceum的5个菌株各聚为一类,每一类又可区分为不同组,以前者分为3组,后者分为2组差异显著,表明种内存在不同的菌系类型;组的划分与菌株致病力及其寄主品种间没有必然的相关性,但与菌株分布的大区域生态气候类型似有联系,供试的东北地区F.graminearum大多数菌株与西北的菌系有较大的遗传相似性,与江苏的菌系相距甚远。
Two populations of Fusarium that caused wheat scab in northeastern China were population analyzed by RAPD and compared with those in Jiangsu and Northwest China. A total of 16 primers were screened for amplification reaction, and their amplification patterns showed that the tested strains were polymorphic among species and species. Most primers amplified a species of characteristic bands, can be used as species identification. Based on the phylogenetic tree of all the phylogenetic trees, 34 strains of Fusarium graminearum and 5 strains of F. avenaceum were clustered into one group, and each group was divided into different groups. The former were divided into three groups , The latter is divided into two groups significant difference, indicating that there are different strains within the species; group division and pathogenicity of the host strains and there is no necessary correlation between the host species, but with large strains of strains of ecoclimatic types Most of F. graminearum strains in northeastern China were closely related to the strains in the northwest of China. They were far away from Jiangsu’s germ line.