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目的探讨砷对动物的毒性作用及中药对其解毒效果。方法用亚砷酸钠毒物染毒小鼠(造模),然后用海尔福口服液(中药制剂)分别按小剂量(治疗1组)和大剂量(治疗2组)灌胃治疗。同时设染毒不治疗组(即模型组)和正常组做对照。实验连续30 d。测定染毒前、后和治疗后的血红蛋白(Hb)。实验结束,从眼球取血,分离血清,测定丙氨酸氨基转移酶(ALT),尿素;处死动物后,取肝脏作病理研究,取大脑制成10%脑匀浆,测定超氧阴离子自由基清除率。结果正常组、模型组、治疗1组、治疗2组,造模前Hb,各组比较(P>0.05)差异无统计学意义;造模后(治疗前)Hb依次分别为143.77±7.11、120.47±14.23、117.66±7.01、125.66±6.86(g/L),正常组明显高于造模的三个组,差异有统计学意义(P<0.01),其中最低为治疗1组,同时造模后的三个组均明显低于其造模前水平,差异有统计学意义(P<0.01)。治疗后Hb分别为152.37±13.69、122.46±12.52、137.62±11.29、151.00±9.09(g/L),模型组最低,明显低于正常组和治疗2组,差异有统计学意义(P<0.05或P<0.01),治疗1组、治疗2组治疗后明显高于治疗前水平,差异有统计学意义(P<0.01),而模型组治疗前后比较,差异无统计学意义(P>0.05)。病理研究结果表明,砷对肝脏有损害作用,模型组肝损害较严重,治疗组较轻。脑对超氧阴离子自由基清除率和肝ALT活性,各组间也有一定变化。结论砷对造血系统有明显的抑制作用和对肝脏有明显损害作用,中药海尔福治疗后恢复明显。
Aim To investigate the toxicity of arsenic to animals and the detoxification effect of traditional Chinese medicine. Methods The mice were treated with sodium arsenite poison (model), and then treated with small dose (treatment group 1) and high dose (treatment group 2) with Haier Fu oral solution (traditional Chinese medicine preparation). At the same time set the no-treatment group (model group) and normal group as a control. Experiment for 30 days. Hemoglobin (Hb) was measured before, after and after treatment. At the end of the experiment, blood was collected from the eye and the serum was separated to determine the alanine aminotransferase (ALT) and urea. After the animals were sacrificed, the liver was taken for pathological study. The brain was made into 10% brain homogenate and the superoxide anion free radical clearance rate. Results There was no significant difference in Hb between normal group, model group, treatment group 1, treatment group 2 and model group before treatment (P> 0.05), Hb values were 143.77 ± 7.11,120.47 ± 14.23, 117.66 ± 7.01, 125.66 ± 6.86 (g / L), respectively, which were significantly higher in the normal group than those in the model group (P <0.01) Of the three groups were significantly lower than the pre-model levels, the difference was statistically significant (P <0.01). Hb after treatment was 152.37 ± 13.69,122.46 ± 12.52,137.62 ± 11.29,151.00 ± 9.09 (g / L), the lowest in the model group was significantly lower than the normal group and the treatment group 2, the difference was statistically significant (P <0.05 or P <0.01). The treatment group 1 and treatment group 2 were significantly higher than those before treatment, the difference was statistically significant (P <0.01), while there was no significant difference in the model group before and after treatment (P> 0.05). Pathological studies have shown that arsenic has a detrimental effect on the liver, liver damage in the model group is more serious, the treatment group lighter. Brain superoxide anion free radical scavenging rate and liver ALT activity, there are some changes between the groups. Conclusion Arsenic has a significant inhibitory effect on the hematopoietic system and a significant detrimental effect on the liver. The treatment with traditional Chinese medicine Herceptin shows obvious recovery.