作者获得了抗HBsAg共同抗原决定簇(a)和亚型特异性决定簇(d、y、w和r)的单克隆抗体。用抗-a包被固相,辣根过氧化物酶分别标记d、y、w和r单克隆抗体。用夹心法检测了美国国立卫生研究院和日本各地收集的不同HBsAg亚型的血清。结果说明该方法特异性高,能准确有效地对HBsAg进行分型。HBsAg的亚型是乙型肝炎病毒(HBV)基因型的表现型。基于两组相互排斥的抗原决 The authors obtained monoclonal antibodies against the HBsAg common antigenic determinant (a) and the subtype-specific determinants (d, y, w and r). The anti-a coated solid phase, horseradish peroxidase were labeled monoclonal antibodies d, y, w and r. Sandwich assays were used to detect sera from different NIH HBsAg subtypes collected by NIH and Japan. The results show that the method is highly specific and can accurately and effectively classify HBsAg. The subtype of HBsAg is a phenotype of the hepatitis B virus (HBV) genotype. Based on two sets of mutually exclusive antigens