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本文报道10例肝细胞型肝癌患者手术切除标本中癌、癌周和远处肝组织的谷胱甘肽含量测定结果。 材料和方法 肝癌患者10例(男9例,女1例),中位年龄54岁(42～60岁)。于手术时分别切取肝癌、癌周和远处肝组织各一块。剪碎后分别用冰生理盐水洗涤5次,除去血液和血块后用滤纸吸干。减量法称取湿重约200mg组织,加冰生理盐水2ml制备匀浆。2000g离心10min后吸取上清液10μl,加入无水乙醇50μl沉淀组织蛋白质。再次以2000g离心5min后吸取上清液20μl,经邻苯二甲醛柱
This article reports the results of determination of glutathione content in cancerous tissue, cancerous tissue, and distant liver tissue in surgical specimens of 10 patients with hepatocellular carcinoma. Materials and Methods There were 10 patients with liver cancer (nine males and one female). The median age was 54 years (42-60 years). At the time of surgery, one liver tissue, one cancerous area, and one distant liver tissue were cut out. After shredding, wash separately with ice physiological saline 5 times, remove blood and blood clots, and blot dry with filter paper. Weigh the wet tissue weighing about 200mg, add 2ml ice physiological saline to prepare the homogenate. After centrifugation at 2000 g for 10 min, 10 μl of the supernatant was aspirated, and 50 μl of ethanol was added to precipitate the tissue protein. After centrifuging again at 2000g for 5 min, the supernatant was pipetted with 20 μl of phthalaldehyde.