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背景:在以往研究中,动脉粥样硬化性脑梗死具有遗传素质倾向及免疫失衡已引起诸多国内外学者的关注,一些从免疫遗传学角度对该病进行的研究发现:动脉粥样硬化性脑梗死与人类的免疫遗传基因多态性有关,尤其是与人类白细胞抗原系统基因-Ⅱ类基因DR关系密切。目的:探讨人类白细胞抗原系统基因-Ⅱ类基因与动脉粥样硬化性脑梗死免疫遗传的相关性。设计:单一样本单因素分析。单位:吉林大学中日联谊医院,哈尔滨市第一医院神经科。对象:实验组为1998-01/2000-12哈尔滨市第一医院动脉粥样硬化性脑梗死住院患者31例,对照组为同期本院体检健康人30人,两组祖籍三代出生地均为中国北方地区汉族人,无血缘关系,均知情同意,并签署同意书。方法:实验于2003-06/12在哈尔滨市第一医院血液研究中心完成。两组分别留取5mL外周血,采用以往方法提取基因组DNA。通过国际互联网在人类基因库中对6号染色体的人类白细胞抗原系统基因取得的单核苷酸进行检索。选择性地合成所需每一DR和DQ等位基因或亚基因引物及反义引物一对,使用美国PE公司生产的顺序特异性引物仪(4800)及有关试剂进行顺序特异性引物-聚合酶链式反应方法特异性地扩增所需检测DNA片断,引物由上海基因研究所协助合成。实验数据采用四格表确切概率计算法,计算相对危险度和确切概率P。主要观察指标:人类白细胞抗原系统基因-DRBI及DQBI等各位基因。结果:病例组人类白细胞抗原系统基因-DRB10301相对危险度RR=5.6842,明显高于其他位点,差异显著(P<0.05);其他人类白细胞抗原系统基因-DRB1和DQB1各等位基因未见异常。结论:人类白细胞抗原系统-DRB10301基因位点可能为北方汉族人动脉粥样硬化性脑梗死的致病易感基因或与其他基因相连锁共同致病。
BACKGROUND: In the past studies, atherosclerotic cerebral infarction with genetic predisposition and immune imbalance has attracted the attention of many scholars at home and abroad, some from the immune genetics study of the disease found: atherosclerotic brain Infarction and human immune genetic polymorphism, especially with the human leukocyte antigen system gene-type Ⅱ DR gene closely. Objective: To investigate the relationship between HLA-Ⅱ gene and immune inheritance of atherosclerotic cerebral infarction in human leukocyte antigen system. Design: Single sample univariate analysis. Unit: China-Japan Friendship Hospital, Jilin University, Harbin First Hospital Department of Neurology. PARTICIPANTS: The experimental group consisted of 31 hospitalized patients with atherosclerotic cerebral infarction in Harbin First Hospital from January 1998 to December 2000. The control group consisted of 30 healthy subjects in our hospital during the same period. The two ancestral home births were both from China Northern Han Chinese, no kinship, informed consent, and sign the consent form. Methods: The experiment was performed at the Blood Research Center of Harbin First Hospital from June 2003 to December 12. Two groups were left in 5mL of peripheral blood, using the previous method to extract genomic DNA. Retrieval of single nucleotide sequences from human leukocyte antigen system gene on chromosome 6 in the human gene bank via the Internet. A pair of each of the DR and DQ alleles or subgenomic and antisense primers required for the synthesis was selectively synthesized, and a sequence-specific primer-Polymerase The chain reaction method specifically amplifies the DNA fragment for detection, and the primer is assisted by the Shanghai Institute of Genomes. The experimental data uses the exact formula of the four-grid table to calculate the relative risk and the exact probability P. MAIN OUTCOME MEASURES: Human leukocyte antigen system genes - DRBI and DQBI. Results: The relative risk of human leukocyte antigen system gene-DRB10301 in case group was RR = 5.6842, which was significantly higher than that in other sites (P <0.05). There were no abnormalities in other HLA-DRB1 and DQB1 alleles . Conclusion: The human leukocyte antigen system-DRB10301 gene may be the causative gene of pathogenicity in atherosclerotic cerebral infarction in northern Han people or linked with other genes.