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目的探讨基质金属蛋白酶2(MMP-2)及抑癌基因PTEN在人脑星形瘤中的表达及二者与人脑星形细胞瘤侵袭性的关系。方法用免疫组织化学SABC法检测50例人脑星形细胞瘤组织和10例正常人脑组织中的MMP-2和PTEN蛋白的表达,并且分析二者与人脑星形细胞瘤临床病理分级的关系。结果 MMP-2和PTEN在低度恶性星形细胞瘤和高度恶性星形细胞瘤组织中表达差别有统计学意义(p<0.05)。随着星形细胞瘤恶性度增高,MMP-2的表达强度呈上升趋势而PTEN表达强度逐渐下降;Spearman等级相关分析表明人脑星形细胞瘤中MMP-2和PTEN之间呈负相关(Rs=-0.518,P<0.01)。结论 MMP-2和PTEN是人脑星形细胞瘤分化程度和转移的潜在生物学指标,联合检测MMP-2和PTEN更有利于判断星形细胞瘤生物学行为和病理分级。
Objective To investigate the expression of matrix metalloproteinase-2 (MMP-2) and tumor suppressor gene PTEN in human brain astrocytomas and their relationship with invasiveness of human astrocytomas. Methods Immunohistochemical SABC method was used to detect the expression of MMP-2 and PTEN protein in 50 cases of human astrocytoma and 10 cases of normal human brain tissue, and to analyze the relationship between them and clinicopathological grade of human astrocytoma relationship. Results The expressions of MMP-2 and PTEN in low-grade astrocytomas and high-grade astrocytomas were significantly different (p <0.05). As the degree of malignancy of astrocytomas increased, the intensity of MMP-2 increased and the intensity of PTEN gradually decreased. Spearman’s rank correlation analysis showed that there was a negative correlation between MMP-2 and PTEN in human astrocytoma (Rs = -0.518, P <0.01). Conclusions MMP-2 and PTEN are potential biomarkers for the differentiation and metastasis of human astrocytoma. Combined detection of MMP-2 and PTEN is more helpful for the determination of the biological behavior and pathological grade of astrocytoma.