【摘 要】
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The aim of this experiment was to investigate whether FSH could regulate the proliferation of calf Sertoli cells and the relationship with CDC25B through Wnt/β
【机 构】
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College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, China
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The aim of this experiment was to investigate whether FSH could regulate the proliferation of calf Sertoli cells and the relationship with CDC25B through Wnt/β-catenin signaling pathway.The experimental method was to culture Sertoli cells with different concentrations of FSH (40,80,120 and 150 ng·mL-1) and to treat the cultured cells with XAV939 (β-catenin inhibitor) and to detect the expression of CDC25B,CDC2,C-MYC and β-catenin.The results of the experiment showed that FSH could promote the proliferation of cells and its effect was good at 80 ng·mL] concentration.FSH could promote the expression of CDC25B,CDC2,C-MYC and β-catenin.The expression of C-MYC and β-catenin in group FSH+XAV939 was lower than that in group FSH.There was no difference in mRNA expression of CDC25B in group FSH and group FSH+XAV939.The protein expression of CDC25B in group FSH+XAV939 was lower than that in group FSH.The conclusion was that FSH could promote the proliferation of calf Sertoli cells through Wnt/β-catenin signaling pathway.CDC25B was upregulated by FSH and CDC2 could promote the proliferation of calf Sertoli cells.The protein level of CDC25B was affected by Wnt/β-catenin signaling pathway.
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