目的　观察去甲二氢愈创木酸 (NDGA)对在体血管生成的影响。方法　采用鸡胚绒毛尿囊膜 (CAM)试验 ,检测NDGA对血管生成的影响。结果　 2 .5× 1 0 -6mmol以上量的NDGA均对血管生成有抑制作用 ,在实验剂量 ( 0 .5～ 5 0 )× 1 0 -6mmol范围内 ,随给药量的增加 ,其抑制作用愈加明显。采用Bouin’s液原位固定后取膜、DAB染色、普通显微镜下观察或用图像分析方法可使结果更具可信性与可比性。结论　NDGA可抑制血管生成 ,对肿瘤及其它病理情况下的抗血管生成治疗可能具有重要的应用价值 Objective To observe the effect of nordihydroguaiaretic acid (NDGA) on in vivo angiogenesis. Methods Chick chorioallantoic membrane (CAM) test was used to detect the effect of NDGA on angiogenesis. The results of 2.5 × 10-6mmol of NDGA are on the inhibition of angiogenesis in the experimental dose (0.5 ~ 50) × 10-6mmol range, with the increase in the amount of its inhibition More obvious. Using Bouin’s solution after in situ fixation, DAB staining, microscopic observation under normal microscope or image analysis method can make the results more credible and comparable. Conclusion NDGA can inhibit angiogenesis and may be of great value in the anti-angiogenic therapy of tumors and other pathological conditions