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为调查我国部分地区规模化蛋鸡场沙门菌生物被膜(BF)与耐药的相关性,2014~2015年从四川、云南、湖北、辽宁、天津、安徽六省规模化蛋鸡养殖场送检的病死鸡分离鉴定出40株沙门菌。玻片凝集法显示分离株包括6种血清型,其中以鼠伤寒沙门菌为主。结晶紫染色法检测生物被膜结果表明,15%(6/40)菌株形成强生物被膜;52.5%(21/40)菌株形成中强生物被膜;32.5%(13/40)菌株不形成生物被膜。K-B纸片法进行药敏试验的结果显示,产BF的27个沙门菌分离株对环丙沙星、诺氟沙星、恩诺沙星、复方新诺明、多西环素、四环素、链霉素、氨苄西林和青霉素9种药物均为多重耐药,耐药率100%,其中对多黏菌素B和头孢噻肟的敏感性显著降低。不产BF的13株沙门菌对以上受试抗生素药物均敏感,耐药率为0。结果表明蛋鸡沙门菌生物被膜与耐药存在相关性。
In order to investigate the correlation between Salmonella biofilm (BF) and drug resistance in large-scale laying hens in some areas in China, from 2014 to 2015, six large-scale laying hens from Sichuan, Yunnan, Hubei, Liaoning, Tianjin and Anhui The dead chickens were isolated and identified 40 strains of Salmonella. The slide agglutination method showed that the isolates included 6 serotypes, of which Salmonella typhimurium was the major one. The results of crystal violet staining showed that 15% (6/40) strains formed strong biofilm, 52.5% (21/40) strains formed medium strong biofilm and 32.5% (13/40) did not form biofilm. KB disk method for drug sensitivity test results show that 27 BF Salmonella isolates of ciprofloxacin, norfloxacin, enrofloxacin, cotrimoxazole, doxycycline, tetracycline, the chain Nine antibiotics, ampicillin and penicillin were multi-drug-resistant with resistance rate of 100%, of which the sensitivity to polymyxin B and cefotaxime was significantly reduced. Thirteen Salmonella strains that did not produce BF were sensitive to the above tested antibiotics, and the resistance rate was 0. The results showed that there was a correlation between Salmonella biofilm and drug resistance.