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莨菪根为镇痛镇静药,含有莨菪碱和东莨菪碱。茛菪根中莨菪碱、东茛菪碱的定量用J P XI法和 HPLC 法测定了10个莨菪根样品中莨菪碱和东莨菪碱的含量,并比较了两种方法的测定值。 1 标准品市售氢溴酸东莨菪碱(C_(17)H_(21)NO_4 HBr ·H_2O,pml05～106℃,和光纯药工业产);1-莨菪碱(C_(17)H_(23)NO_3,mp 108～108.5℃,东京化成工业产)。 2 JPXI定量法用该法测定了10g样品(通过48目筛,60℃下干燥8h)中生物碱含量。 5 HPLC定量法样品溶液:精密称取200mg样品于具塞离心管内,加2.0ml 70％甲醇用超声波在室温下处理20min后以3000次/min速度离心5min。取上清液,并用
Radix is an analgesic sedative that contains scopolamine and scopolamine. Quantitative determination of scopolamine and scopolamine in roots of Japonica roots The contents of scopolamine and scopolamine in 10 Japonica root samples were determined by J P XI method and HPLC method, and the values measured by the two methods were compared. 1 Standard products Commercially available scopolamine hydrobromide (C 17 H 21 NO 4 HBr H 2 O, pml 05-106°C, produced by Wako Pure Chemical Industries); 1-Strontium base (C 17 H 23 NO 3 ) Mp 108 to 108.5°C, Tokyo Chemical Industry Co., Ltd.). 2 JPXI quantification method The alkaloid content in a 10 g sample (48 mesh, dried at 60°C for 8 hours) was measured by this method. 5 HPLC quantification sample solution: Accurately weigh 200 mg of the sample in a stoppered centrifuge tube, add 2.0 ml of 70% methanol and centrifuge at room temperature for 20 min and centrifuge at 5 000 min/min for 5 min. Take the supernatant and use