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目的:建立HPLC测定解毒祛瘀滋肾方中异阿魏酸含量的方法,为其质量控制提供依据。方法:采用高效液相色谱法对解毒祛瘀滋肾方异阿魏酸的含量进行测定,液相色谱柱碳18柱,流动相为乙腈-0.1%磷酸(13∶87),流速1.0mL.min-1,柱温25℃,检测波长为316nm。结果:异阿魏酸含量测定的线性范围0.475～3.800μg,相关系数R为0.9995,加样回收率为98.77%。结论:该法快速、简便、准确,能有效控制该方的质量。
Objective: To establish a HPLC method for the determination of isoferulic acid in Jiedu Quyu Zishen Recipe, and provide a basis for its quality control. Methods: HPLC was used to determine the content of isoferulic acid in detoxification, blood stasis and nourishing kidney. The mobile phase consisted of acetonitrile-0.1% phosphoric acid (13:87) with a mobile phase of 1.0 mL. min-1, column temperature 25 ℃, detection wavelength of 316nm. Results: The linear range of isoferulic acid was 0.475 ~ 3.800μg, the correlation coefficient R was 0.9995, the recovery rate was 98.77%. Conclusion: The method is fast, simple and accurate, which can effectively control the quality of the side.