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为了从分子水平上检验野生棉遗传特性向陆地棉转育的结果以及育成种质之间的遗传差异，通过等电聚焦电泳和RAPD技术，对来自科遗181陆地棉品系与野生比克氏棉杂交后代的6个遗传稳定的不同种质系及其亲本的过氧化物酶同工酶图谱和DNA指纹图谱进行了分析。结果如下：（1） 6个种质系的基本酶谱特征均倾向于陆地棉亲本，但在其中2个种质系的过氧化物酶图谱中，观察到各具有1条等电点值（PI）为4.85的野生亲本的特征带；（2） DNA指纹图谱的分析表明，不同种质系之间在基因组水平上具有高度的异质性。并且在OPO10和OPO11 2个引物的扩增图谱中观察到4个育成种质系具有野生比克氏棉亲本的特征带。
In order to examine the genetic characteristics of wild cotton transferred to upland cotton at the molecular level as well as the genetic differences between breeding germplasm, isoelectric focusing (AFLP) Peroxidase isoenzyme patterns and DNA fingerprints of 6 genetically stable different germplasm lines and their parents were analyzed. The results are as follows: (1) The basic zymogram characteristics of six germplasm lines tended to be upland cotton parents, but in the peroxidase map of two germplasm lines, one isoelectric point value ( PI) was 4.85. (2) The analysis of DNA fingerprinting showed that there was a high degree of heterogeneity at genome level among different germplasm lines. Four bands of breeder germplasm with wild-type Becker’s cotton parents were also observed in the amplification profiles of two OPO10 and OPO11 primers.