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从17个国家引进331个黑杨派无性系(其中52个美洲黑杨无性系),在山东省长清县营建我国第一个黑杨派无性系基因库。连续10年对基因库内美洲黑杨无性系进行了多性状系统研究,结果表明各无性系间在物候期、生长、生根、抗寒和抗病虫等方面均存在着显著的差异,遗传变异丰富;系间的主要性状与无性系起源纬度及各性状间存在一定相关性。主要材料性状变异的研究结果表明,38个8年生美洲黑杨无性系在木材基本密度、纤维长度无性系间变异达到极显著水平;木材密度和纤维长度与树高和胸径呈一定相关性;木材密度和纤维长度这两个性状在遗传上相互独立,受不同遗传机制控制。利用分子遗传标记RAPD技术研究库内美洲黑杨无性系DNA多态性,结果表明本库美洲黑杨DNA多态率为86%,再次证明本库美洲黑杨遗传多样性高,可为我国杨树改良提供丰富的育种材料。
331 black poplar clones were introduced from 17 countries (of which 52 Populus clippinus clones), and the first black poplar clones in China were built in Changqing County, Shandong Province. The results showed that there were significant differences in phenology, growth, rooting, cold tolerance and pest and disease resistance between clones in all clones. Genetic variation There is a certain correlation between the main characters of the lines and the origin latitude and the traits of the clones. The results of the variation of the main material traits showed that 38 eight-year-old poplar clones had significant variations in the wood basic density and fiber length clones; wood density and fiber length had some correlation with tree height and DBH; wood The two traits, density and fiber length, are genetically independent of each other and are controlled by different genetic mechanisms. The molecular genetic marker RAPD technology was used to study the DNA polymorphism of Populus nigra in clones. The results showed that the DNA polymorphism of Populus nigra was 86%, which proved again that Populus nigra Poplar has high genetic diversity, Tree improvement provides abundant breeding material.