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目的:对江苏省2009年分离出的伤寒沙门菌进行同源性分析。方法:用生化和血清学方法,对江苏省2009年从肠道传染病患者中分离到的伤寒沙门菌进行鉴定。伤寒沙门菌基因组经限制性内切酶XbaI酶切后,采用脉冲场电泳获得电泳图谱,再利用BioNumerics软件对电泳图谱进行同源性分析。结果:共从江苏省9个地区分离得到39株伤寒沙门菌,BioNumerics分析结果显示,共有31个不同的PFGE带型出现,除5株以外,其余34株分布于8个相似性在85%以上的簇内。结论:江苏省2009年可能存在8个主要的伤寒沙门菌克隆,这些克隆传播可能是同期伤寒流行的主要病原。PFGE指纹图谱为伤寒沙门菌分子分型网络监测打下了良好的基础,为疾病主动监测和传染来源追踪提供了技术支持。
Objective: To analyze the homology of Salmonella typhi isolated in 2009 in Jiangsu Province. Methods: The biochemical and serological methods were used to identify Salmonella typhi isolated from patients with intestinal infectious diseases in Jiangsu Province in 2009. Salmonella typhi genome restriction endonuclease XbaI digestion, the use of pulsed-field electrophoresis electrophoresis map, and then use BioNumerics software for electrophoretogram homology analysis. Results: Thirty-nine Salmonella typhi strains were isolated from 9 districts of Jiangsu Province. BioNumerics analysis showed that there were 31 different PFGE patterns. Except for 5 strains, the remaining 34 strains were distributed in 8 similarities above 85% Within the cluster. Conclusion: There may be eight major Salmonella typhi clones in 2009 in Jiangsu Province. These clones may be the main pathogen of typhoid epidemics in the same period. PFGE fingerprinting laid a good foundation for the molecular typing of Salmonella typhi and provided technical support for active disease surveillance and source tracking of infectious agents.