论文部分内容阅读
为研究实验性变态反应性神经炎(Experimentalalergicneuritis,ENA)和格林-巴利综合征(GBS)的发病机理,寻找GBS的有效诊断、治疗方法,本实验采用反复超速离心、稀盐酸等电点沉淀、CM-22弱酸性阳离子交换层析等方法,从牛硬脊膜内马尾神经根中提取到高纯度髓鞘碱性蛋白Ⅱ(MyelinBasicProteinⅡ,P2)。鉴定结果表明,该蛋白的分子量为15000,pl=9.5,在SDS-PAGE垂直板电泳、PAGE圆盘电泳中显示一条蛋白区带,在双向免疫扩散、微量免疫电泳中,与进口标准抗牛P2血清之间形成一条沉淀线,HPLC测定氨基酸成份与Kad-lubowski报告的国外四个实验室测定的结果大致相同,具有诱导EAN的生物活性。
In order to study the pathogenesis of experimental allergic neuritis (ENA) and Guillain-Barre syndrome (GBS) and to search for an effective diagnosis and treatment of GBS, this experiment used repeated ultracentrifugation, dilute hydrochloric acid isoelectric point precipitation , CM-22 weak acid cation exchange chromatography and other methods, high purity myelin basic protein Ⅱ (Myelin Basic Protein Ⅱ, P2) was extracted from the cauda equina nerve roots. The identification results showed that the molecular weight of the protein was 15000, pl = 9.5, showing a protein band in SDS-PAGE vertical plate electrophoresis and PAGE disc electrophoresis. In the two-way immunodiffusion and microimmunoelectrophoresis, A serum line formed between cattle P2 serum. The amino acid composition by HPLC was roughly the same as the result of four laboratory tests reported by Kad-lubowski, and had the biological activity of inducing EAN.